Chitraka is an image analysis & management tool for semi-automatic recognition and quantification of expressed gene spots from microarray experiments. Chitraka provides automatic grid layout, spot finding, comprehensive spot statistics and robust background signal correction. Chitraka traces spots accurately and automatically. It detects doughnut shaped spots leading to enhanced accuracy in detecting differential expression. It is compatible with any data analysis software. Chitraka is a stand-alone tool that runs on Windows platform. It can also be customized to run with any specific scanner.

  Key Benefits:

Multiple Scanner Acquisitions
Import images from multiple scanners from different vendors.

Image Formats
Full support for 16-bit grayscale TIFF images

Multiple Channel Experiments
2-channel image analysis(Cy3 and Cy5)

Imaging Operations
Quality control of images to reduce skewing/rotation. Image enhancement for contrast, brightness,sharpness.Multiple-zoom levels.

Array Lists
Support for arraylist files such as GAL.

Quantification
Automatic spot segmentation and accurate spot boundary identification. Automatic doughnut spot detection and compensation.

Gridding
Automatic fine tuning of level one grids ( eliminates manual errors for accurate grid definitions).Automatic fine tuning of level two grids. (eliminates manual errors for accurate subgrid definitions).

User Interface
Grid by grid view for convenient block navigations in large multi-level array lists.Workspaces to work with multiple simultaneous images from different experiments and statistical data.

Data Views
Automatically generates Scatter Plots/Histograms of any analysis measurements, including background corrected and standard deviations.